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. 2006 Dec 19;96(1):126–133. doi: 10.1038/sj.bjc.6603529

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Copyright © 2007 Cancer Research UK

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Inhibition of endogenous Bmi-1 causes replication arrest in SCC4 cells. (A) Rapidly proliferating SCC4 cells were infected with LV-GFP or LV-Bmi-1i. After 3 or 10 days, the images were obtained for GFP revealing those cells that were originally infected by the viral vectors. Original magnification, × 100. (B) Three days after infection of NHOK (05-10) and SCC4 cells with LV-GFP or LV-Bmi-1i, the level of Bmi-1 transcript was measured by semi-quantitative RT–PCR. The extent of Bmi-1 knockdown by LV-Bmi-1i was quantitated by Scion Image software against those of GAPDH amplification.