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. 2007 Oct 16;97(10):1372–1380. doi: 10.1038/sj.bjc.6604019

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Copyright © 2007 Cancer Research UK

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Extracellular nucleoside diphosphate kinase (NDPK) and EC P2Y₁ receptor activation induce angiogenesis in vitro. (A and B) semi-pure as well as purified bovine liver NDPK induced CD31⁺ cell tubulogenesis over 24 h. Statistical significance versus control was determined using the Mann-Whitney test. (C) NDPK-B derived from 435S cell-secreted NDPK extract promoted in vitro angiogenesis over 72 h at various depletion levels. Control C mean=2645.9 angiogenesis units. (D) 24 h incubation with ATP, 2MS-ATP, and NDPK extract all stimulated angiogenesis; blocked by MRS2179. Control D mean=6183.8±1840.4 angiogenesis units. (E) 72 h incubation with ATP and NDPK extract both promoted angiogenesis; blocked by P2Y₁R antagonist, MRS2179. Control E mean=1256.6±240.9 angiogenesis units. Controls consisted of CD31⁺ cells incubated in CDMEM supplemented with 2% FBS. The angiogenic stimulation control used was endothelial growth media-2 (EGM-2™) containing VEGF.