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. 2007 Oct 30;97(10):1457–1464. doi: 10.1038/sj.bjc.6604055

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Copyright © 2007 Cancer Research UK

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Overview of the MS-MLPA procedure (Nygren et al, 2005). The gene-specific probes spanning a recognition site for the restriction enzyme Hha1 are hybridised to the target DNA and subsequently ligated and digested with the methylation-sensitive enzyme Hha1. Undigested probes, that is probes of which the recognition sequence is methylated, will be amplified. If the CpG site is unmethylated, the DNA/probe complex will be digested and no amplification will take place. For each DNA sample, the MS-MLPA was performed with and without Hha1 digestion.