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. 2007 Jun 26;97(2):210–217. doi: 10.1038/sj.bjc.6603857

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Copyright © 2007 Cancer Research UK

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The average tumour volumes of mice bearing established tumours after treatment with three vaccines. A/J mice were engrafted with 10⁶ unmodified Neuro-2A cells and vaccinated with Neuro-IL2/IL12 or AJ-IL2/IL12 cells or RPMI medium on days 3, 5 and 7 directly into the tumour. By 12 days post engraftment, mice vaccinated with Neuro-IL2/IL12 or AJ-IL2/IL12 (n=16 per group) had significantly smaller tumours compared to RPMI-treated controls (n=6). Day 12 is the point when all the control mice have to be killed due to their tumour size. Significance was determined by t-test (^***P<0.001).