Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jun 26;97(2):210–217. doi: 10.1038/sj.bjc.6603857

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ELISpot and LDH release assays were performed using splenocytes from mice with treated established tumours. Mice were engrafted subcutaneously with tumour cells, then vaccinated 3, 5 and 7 days later. On day 12, six mice from each group were culled and splenocytes coincubated with Neuro-2A cells for 48 h hours. (A) Splenocytes from mice vaccinated with Neuro-IL2/IL12 or AJ- IL2/IL12 had significantly greater numbers of IL-2-expressing splenocytes than RPMI-vaccinated controls in response to Neuro-2A target cells (^**P<0.01). Photographs are of representative wells and significance was determined by t-test. (B) LDH assay determined the cytotoxicity of splenocytes against Neuro-2A targets using a 27 : 1 E : T ratio. Mice vaccinated with Neuro-IL2/IL12 or AJ-IL2/IL12 showed significantly greater toxicity against Neuro-2A targets than splenocytes from mice vaccinated with RPMI. Significance was determined by t-test (^*P<0.05).