Figure 2.

HO-3 binds within EGF-like domain I of EpCAM. (A) HEK293 were transiently transfected with wild-type EpCAM or the EpCAM EGF-I mutant and stained with HO-3 (grey curves; upper panels) or C215 (grey curves; lower panels) in combination with a PE-conjugated secondary antibody. As a control, primary Ab was omitted (black line). (B) Transfections were performed as in (A), proteins were separated by 10% SDS–PAGE, and EpCAM was visualised with the mAb HO-3. For a control, levels of actin were assessed on the same membrane. Shown are the representative results from three independent experiments.