Table 2. Identification of EpCAM peptides recognised by HO-3 and C215.
| AA position | Peptide sequence | Signal intensity (BLU) | mAb |
|---|---|---|---|
| 49–70 | TSVGAQNTVICSKLAAKCLVMK | 6829 | HO-3 |
| 67–88 | LVMKAEMNGSKLGRRAKPEGAL | 18813 | HO-3 |
| 175–196 | QLDPKFITSILYENNVITIDLV | 3543 | HO-3 |
| 31–52 | NYKLAVNCFVNNNRQCQCTSVG | 16703 | C215 |
| 103–124 | GLFKAKQCNGTSTCWCVNTAGV | 12006 | C215 |
Screening a peptide library covering the complete ECD of EpCAM showed that the listed peptides had the strongest interaction with the indicated antibody. Signals ranging from 30 to 800 Boehringer Units (BLU) were considered to be non-specific (background).