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. 2007 Jul 31;97(5):637–645. doi: 10.1038/sj.bjc.6603918

Figure 4.

Figure 4

TUNEL assay in activated lymphocytes after co-culturing with OvCa3 or A2780 cells. (A–F) Representative TUNEL assay photos of PBL after 24 h culturing in various conditions. Activated PBL cultured alone for 24 h showed only limited apoptosis (A). In the presence of 10 nM CRH, PBL apoptosis was moderately increased (B). Similarly, moderate levels of PBL apoptosis occurred after co-culturing with OvCa3 or A2780 cells (C). Activated PBL cultured with OvCa3 or A2780 cells primed with 100 nM CRH for 48 h, then with the continuous presence of 10 nM CRH for 24 h, showed high levels of apoptosis (D). Activated PBL cultured with OvCa3 or A2780 cells primed with 100 nM CRH+1 μM antalarmin for 48 h, then with 10 nM CRH+100 nM antalarmin for 24 h, reverted the effects of CRH (E). Similarly, addition of an anti-Fas blocking antibody in co-cultures with CRH-treated cells, reverted the effects of CRH (F). (G) Quantitation of results. Data represent mean±s.e. (n=4) (*P<0.05).