Table 5. Mapping the adjacent genomic regions deleted when PTEN is lost using BAC DNA probes spanning the predicted deletion interval on chromosome 10q.
| TMA | Centromeric to PTENa | 5′ flanking PTENb | PTEN c | Telomeric to PTENd | Telomeric to PTENe | Predicted deletion |
|---|---|---|---|---|---|---|
| 19 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 29 | +/− | +/− | +/− | +/+ | +/+ | Small |
| 35 | +/− | +/− | +/− | +/+ | +/+ | Small |
| 38 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 39 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 41 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 46 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 49 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 91 | +/+ | +/− | +/− | +/− | +/+ | Large |
| 184 | +/+ | +/− | +/− | +/+ | +/+ | Microdeletion |
| 28 | +/− | −/− | −/− | +/− | +/− | Microdeletionf largeg |
| 72 | +/+ | −/− | −/− | +/+ | +/+ | Microdeletion |
| 88 | +/− | −/− | −/− | +/− | +/− | Microdeletionf Largeg |
Abbreviations: BAC, bacterial artificial chromosome; FISH, fluorescence in situ hybridisation.
The summarised FISH analysis described the findings from the largest clonal cell population.
+/+ no copy change; +/− hemizygous deletion; −/− homozygous deletion.
Estimated microdeletion: ∼200 kb.
Estimated small deletion: ∼7–8 Mb.
Estimated large deletion: ∼19–21 Mb.
a
10q23.2 (88.2–88.6 Mb).
b
10q23.2 (89.4–89.5 Mb).
c
PTEN locus (89.6–89.7 Mb).
d
10q23.33 (96.2–96.6 Mb).
e
10q25.1 (108.9–109.3 Mb).
f
First event.
g
Second event.