Table 2. Immunohistochemical, cytogenetic and molecular data.

Patients	EGFR: IHC	EGFR: FISH	K-Ras: status	PTEN: IHC
1	1+	A	WT	Pos
2	2+	A	13GaC	Neg
3	3+	P	12GcT	Pos
4	1+	P	WT	Neg
5	3+	P	12GcT	Pos
6	2+	P	12GaT	Neg
7	2+	P	WT	Neg
8	3+	A	WT	Pos
9	3+	A	WT	Pos
10	2+	P*	WT	Pos
11	1+	E	13GaC	Neg
12	3+	E	12GaT	Pos
13	3+	A	WT	Pos
14	3+	P*	WT	Neg
15	2+	A	12tGT	Neg
16	2+	P	WT	Pos
17	1+	A	WT	Pos
18	2+	P*	12GaT	Pos
19	3+	P*	WT	Neg
20	3+	P	13GaC	Pos
21	3+	P	12GaT	Pos
22	1+	E	WT	Pos
23	3+	P	WT	Neg
24	3+	P	WT	Neg
25	3+	A	WT	Pos
26	2+	P*	WT	Pos
27	2+	P	WT	Neg
Total	5/27 (19%) score 1+	8/27 (30%) A	17/27 (63%) WT	16/27=59% Pos
	9/27 (33%) score 2+	16/27 (59%) P	10/27 (37%) Mutated	11/27=42% Neg
	13/27 (48%) score 3+	3/27 (11%) E

Abbreviations: A, EGFR gene amplification; P, chromosome 7 polysomy; P^*, polysomy with rare cells showing EGFR amplification; E, eusomy; WT, wild type; Pos, positive; Neg, negative.