Table 1. hC4.4A expression in human cancer cell lines (flow cytometry).
C4.4A expression
| ||||||||
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Pancreatic cancer | Intensitya | Colorectal cancer | Intensitya unstimulated | Intensitya stimulatedb | Breast cancer | Intensitya | Prostate cancer | Intensitya |
Colo357 | +++ | SW480 | + | + | MCF-7 | +++ | LNCaP | +++ |
Capan2 | ++ | Colo205 | + | + | MELN | +++ | Du145 | +++ |
Panc89 | ++ | HT29 | − | + | HCC1937 | ++ | PC3 | + |
BxPC3 | + | WIDR | − | + | BT47D | ++ | ||
MiaPaca2 | + | Colo320 | − | + | MDA-MB436 | + | ||
Panc1 | − | Colo320DM | + | + | ||||
8.18 | − | SW707 | − | + | ||||
Capan1 | − | SW948 | ++ | ++ | ||||
Lovo | ++ | ++ |
Flow cytometry data were analysed according to the increase in the mean fluorescence intensity as compared to the negative control (normal rabbit IgG plus anti-rabbit IgG-PE): –(negative), intensity 1.0- to 1.5-fold; + (distinct), intensity 1.5- to 4-fold; ++ (strong), intensity 4- to 10-fold; +++ (very strong), intensity >10-fold.
Cells were starved overnight and thereafter incubated for 24 h in RPMI 1640 containing 10% fresh AB0 serum.