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. 2007 Sep 25;97(8):1099–1105. doi: 10.1038/sj.bjc.6604003

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Copyright © 2007 Cancer Research UK

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Effect of CoPP and ZnPP in bortezomib-induced cell death. TaY cells were cultured with CoPP or ZnPP in the presence of bortezomib. (A) The percentage of cell viability was plotted with respect to untreated cells. (B) Apoptosis was expressed as fold increases of annexin V-positive cells in bortezomib-treated cells compared to those in untreated cells. (C) Fold induction (or reduction) of HMOX gene expression measured by real-time RT–PCR. (D) Western blotting of HMOX-1 and GAPDH. Fold increases were expressed as a signal of HMOX-1/GAPDH in the specimen with respect to those in untreated cells. (E) HIF-1 alpha expression in bortezomib-treated TaY cells (real-time RT–PCR, left; western blotting, right).