Figure 6.

Effect of adenovirus-mediated over-expression of p16INK4A on thymidylate synthase (TS) expression and cell-cycle progression. (A) Cell-cycle distribution 24 h after infection of HCT116 cells with adenoviruses encoding Lac Z and p16INK4A at multiplicity of infections (MOIs) of 10 or 25. Cell-cycle distribution was determined by flow cytometry as indicated in Figure 1. Data shown are the average of two independent experiments. (B) Western blot analysis of HCT116 cells 24 h after infection with CA35 or p16INK4A adenoviruses at MOIs of 10 or 25. (C) Cell-cycle distribution following 24 h after infection of MCF-7 cells with adenoviruses encoding Lac Z and p16 at MOIs of 10 or 25. Cell-cycle distribution was determined by flow cytometry as in Figure 1. Data shown are the average of two independent experiments. (D) Western blot analysis of MCF-7 cells 24 h after infection with CA35 or p16INK4A adenoviruses at MOIs of 10 or 25.