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. 2006 Aug 22;95(6):735–743. doi: 10.1038/sj.bjc.6603299

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Copyright © 2006 Cancer Research UK

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Effect of hypoxia on EMAP-II expression in CRC in vitro. (A) Western blot of extracts of DLD-1 (Lanes 1–4 and 9–12) and HT29 cells (Lanes 5–8 and 13–16). Cells were grown in control or hypoxia for 4 and 24 h. Lanes 1, 3, 5, 7, control cell lysates at 4, 24 h; Lanes 9, 11, 13, 15 conditioned media from the same cells; Lanes 2, 4, 6, 8, lysate from hypoxic cells for 4, 24 h; Lanes 10, 12, 14, 16 conditioned media from hypoxic cells. (B) ELISA of conditioned medium from DLD-1 for soluble EMAP-II. Cells exposed to hypoxia for 4 and 24 h. Data represent mean of three determinations±s.d. (C) Flow cytometric determinations of cell surface EMAP-II expression in hypoxia. Untreated DLD-1 and HT29 tumour cells without primary antibody (dashed lines); untreated cells incubated with preimmune antibodies (dotted lines); control cells incubated with R2B2 antibodies against EMAP-II (thin lines); hypoxic cells incubated with R2B2 antibodies against EMAP-II (thick black lines).

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