Figure 4.

EMAP-II-Induced apoptosis of lymphocytes requires cell–cell contact. (A) Experimental design. Tumour cells and Jurkats were cocultured in contact and noncontact conditions. In the noncontact cocultures, tumour cells were plated at the bottom of a culture-plate well and Jurkats at the bottom of a culture insert. The culture insert was placed in the well and the cells were then incubated. In the contact cocultures, tumour cells and Jurkats were plated at the bottom of the same culture-plate well. (B) A=Jurkats were cultured with tumour cells, B=Jurkats+tumour cells pretreated with TNF-α/IFN-γ, C=Jurkats+tumour cells pretreated with TNF-α/IFN-γ and R2B2. Results represent the mean of three determinations±s.e.m. ^* Indicates significant deviations from control values, P<0.05.