Table 1. Primer sequences for the PCR of the 21 exons of the TOP1 gene.

SNP	Localisationa	Primer sequence	Exon length (bp)	Amplicon length (bp)
5′ UTR	G4709344T rs6072250	S: GATACCAGCTCTGCCCAAGA As: AGACTCCAGAAACGGCTGAG	—	656
Exon 1		S: CCGACGTGTTGTTTAAAAG As: CCTAGCGAGCCGATCTAC	279	474
Exon 2		S: GGCCGCGAAGTTACAGTT As: ACGCCATGTCTGTCCTCT	25	285
Exon 3		S: TGTCAAGGTAGGCATACAGA As: GCTGAATGATGAGGGACTT	97	201
Exon 4		S: GGCTAACGCTTTGTGACTTA As: CCTTCCTCCCCTACTAGAAA	124	184
Exon 5		S: CCAGGAACTGATGCTACATA As: TTGGGTTAACTCAGACAACA	56	233
Exon 6		S: CACTCTGACCAGCAATTTTT As: AGTTCAGAGAAATCAGTCCA	96	280
Exon 7		S: TGTATTCATGTTCCCCTTTC As: AGCCCCAAAGGACCTAAG	76	130
Exon 8		S: GCTTTCTCACCATGTTTCTT As: ATTTTAACCCAGTGCTGAGA	107	158
Exon 9	G4774052A (Gly214Ser) rs6029542	S: ATCACTAAATGAGGCTGTGC As: TCTATTACATAGGATCCTTCCTT	116	242
Exon 10		S: TCACTTTTTGGAACCACTTT As: ACTTGATTGTTTTGCAAGGT	122	201
Exon 11		S: TCCCCATTTTCTTTTGACTA As: ATTCCAGAATTCTCCAGAGG	123	184
Exon 12	G4781682A (Arg349Arg) rs6029545	S: TCAGACTTTCCTCTACCTTGA As: CTTTTTGGCCCATAGGAT	188	325
Exon 13	Gly418Lys	S: TTTCACTATCCTCGTGCTCT As: AGGTTATCAGCACCATGAAC	145	223
Exon 14		S: TGCCTGGCTATATTCAAAGT As: GATGGGAAGATGCTCTCAC	144	238
Exon 15	G4795621A (Glu517Lys) rs6129757	S: ATGTCTCTTCCATTCATGCT As: ACCCATGCTTGTTGAATAAT	186	288
Exon 16		S: TGCAAGTTTCTTTAAGTTTGG As: TTCAACATTGTGTGCTTCAT	69	181
Exon 17	G4797898A (Thr591Thr) rs1061982	S: TTCCTCTTTCCCTAACTTCC As: ATCTGGCCAAGCAATACTTA	115	276
Exon 18		S: GATAAGAGCCAGCAAAATCA As: TCCTCTCATTCGCAGATAAT	128	300
Exon 19	T4803328C (Lys676Lys)	S: CATCTTGGTTTCACCTTCTC As: GCTGCACACTTTTCTCTACC	95	222
Exon 20	A4803680G (Asn722Ser)	S: GGTTCTTGAGGACTTTGCTA As: AAGGAGGGCTCAACACTTAC	150	199
Exon 21	G4804749A (splice site) rs6072277	S: CATTGCTGAGTCACCCTAAT As: AGTGAGGCTCAGTTTATCCA	107	224
	G4804829C (Met758Ile) rs12057885

^aThis refers to mutations or SNPs mentioned in databases and that have been especially sought.