Table 1. Plasmids used in this study.

Plasmid	Features	Reference
pMTL20E	Source of ermB	(Oultram et al, 1988)
pMTL4	Shuttle vector backbone	(Chambers et al, 1988)
pET21b	Expression vector	Novagen
pIMP1	pIM13 replicon	(Mermelstein et al, 1992)
pMTL6341aLS2	Clostridial expression cartridge	(Carter et al, 2005)
pMTL20	Cloning vector and source of LacZα
pMTL30	Source of OriT	(Williams et al, 1990)
pCR2.1-TOPO	TA PCR cloning vector	Invitrogen
pCR-Blunt II-TOPO	Blunt PCR cloning vector	Invitrogen
pCR4-TOPO∷fac2	Source of fac2 promoter	Entelechon GmBH
pMTL4-Em	Vector backbone – ColEI Gram-negative replicon+ermB	This study
pMTL552	pMTL4E+pIM13 replicon	This study
pMTL553	pMTL552+expression cartridge	This study
pMTL554	pMTL553+OriT	This study
pEOriT	OriT from pMTL30	This study
pMTL555	pMTL554+LacZalpha	This study