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. 2006 Oct 3;95(9):1202–1211. doi: 10.1038/sj.bjc.6603369

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Copyright © 2006 Cancer Research UK

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Expression of BMI-1 and EZH2. (A) Immunohistochemical staining for BMI-1 expression on biopsy sections taken from different carcinomas and tumour cell line cytospins using the 229F6 monoclonal antibody. (B) Reverse transcription–PCR using EZH2-specific primers of various cell lines: 1, prostate cancer cell line (LNCaP); 2, normal human fibroblasts; 3, lymphoblastoid cell line(LCL)1; 4, LCL2; 5, LCL3; 6, LCL4; 7, cervical cancer cell line (Caski); 8, hepatoblastoma cell line (HepG2); 9, breast cancer cell line (MCF7); 10, breast cancer cell line (BT549); 11, breast cancer cell line (nDA-MB 436); 12, water.