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. 2006 Oct 3;95(9):1202–1211. doi: 10.1038/sj.bjc.6603369

Figure 2.

Figure 2

Interferon-γ production by PBMC and TIL cultures from cancer patients and normal donors following nucleofection with plasmid DNA expressing BMI-1 and EZH2 proteins. Peripheral blood mononuclear cells samples from patients with a variety of (A) epithelial tumours and (C) normal donors were nucleofected with 5 μg of plasmid DNA (pAdTrack-CMV) encoding either BMI-1 and GFP (░), or EZH2 and GFP (▪) or GFP alone (□). (B) Results from paired cultures of TILs and PBMCs from a patient with hepatocellular carcinoma are also shown. Interferon-γ production was measured in the supernatants of serially diluted PBMC cultures using an ELISA. The results shown are means of triplicate wells and standard errors were always less than 10%.