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. 2006 Oct 3;95(9):1202–1211. doi: 10.1038/sj.bjc.6603369

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Copyright © 2006 Cancer Research UK

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Detection and enrichment of IFN-γ secreting cells from normal donors. Peripheral blood mononuclear cells obtained from normal HLA-A0201-positive laboratory donors were stimulated in vitro overnight with the HLA-A0201-restricted EZH2 peptide YMCSFLFNL (aa 666–674) at 10 μg ml⁻¹. Interferon-γ-secreting cells were detected using a PE-labelled IFN-γ detection antibody, enriched on anti-PE magnetic microbeads and selected on automated columns. The figure shows flow cytometric analysis of pre- and postselected samples (stained using an anti-human CD8-PC5 antibody) from the same donor. Dead cells have been gated out after staining with propidium iodide.