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. 2006 Oct 3;95(9):1202–1211. doi: 10.1038/sj.bjc.6603369

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Copyright © 2006 Cancer Research UK

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Staining of normal PBMCs with theYMCSFLFNL (aa 666–674) tetramer. Peripheral blood mononuclear cells isolated from an HLA-A0201-positive normal laboratory donor were stained with a tetramer incorporating the HLA-A0201-restricted EZH2 epitope YMCSFLFNL (aa 666–674) and an FITC-conjugated surface CD8 antibody directly ex vivo (A) and following a 14-day in vitro restimulation step with the YMCSFLFNL (aa 666–674) peptide (B). Peptide restimulation was also carried out on the same population of PBMCs after depletion of CD25⁺ T cells (C). The values shown are the percentage of CD8+ T cells staining positive with the tetramer.