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. 2006 Oct 24;95(10):1348–1353. doi: 10.1038/sj.bjc.6603434

Figure 2.

Figure 2

(A) Activation of caspase-3 after E-4IB and/or cisplatin treatment in A2780 cells. The cells were exposed to E-4IB (5 μM) for 3 h followed by cisplatin (5 or 10 μM). Caspase-3 activity was measured using fluorogenic substrate Ac-DEVD-AFC. (B) Modulation of Ψm. Caspase-3 inhibitor (Z-DEVD) was added to culture medium for 1 h before the exposure of the cells to 2.5 or 5 μM E-4IB for 3 h followed by cisplatin (5 or 10 μM, for 3 or 24 h). (C) Effect of Z-DEVD on the sub-G1 population in the cells treated by E-4IB and/or cisplatin. Sub-G1 was calculated by flow cytometry. The results are representative of at least three independent experiments.