Table 1. Effect of E-4IB on cisplatin-induced clonogenic survival.
|
Concentration (μM)
|
Relative average | |
|---|---|---|
| E-4IB | Cisplatin | Count |
| — | — | 1.00±0.00 |
| 0.5 | — | 0.91±0.06 |
| 1 | — | 0.79±0.09 |
| — | 0.1 | 0.74±0.05* |
| 0.5 | 0.1 | 0.64±0.11 |
| 1 | 0.1 | 0.40±0.02# |
| — | 0.25 | 0.43±0.08** |
| 0.5 | 0.25 | 0.32±0.02 |
| 1 | 0.25 | 0.10±0.09# |
E-4IB=ethyl 4-isothiocyanatobutanoate.
A2780 cells were treated with 0.1 or 0.25 μM cisplatin in the presence or absence of 0.5 or 1 μM E-4IB. Clonogenic survival was assessed by Giemsa-Romanowski staining after 7 days and colony numbers per well of a six-well plate are indicated. Statistical significant differences from the controls, *P<0.05, **P<0.01, and from the corresponding cisplatin-treated samples, #P<0.05.