Figure 3.

RNA encoding Dsc2 is unchanged, whereas that encoding Dsc1 and Dsc3 is increased in sporadic adenocarcinoma. (A) Semiquantitative RT–PCR was used to measure RNA in matched specimens of histologically normal tissue and tumour material. In each case, PCR products were of the expected size and identical in mobility to those amplified from reverse transcribed RNA from normal human epidermal keratinocytes (not shown). To confirm the presence of Dsc1 message in colorectal cancer samples, amplified DNA was blotted onto nitrocellulose and probed with a Dsc1-specific oligonucleotide (predicted to hybridise within the PCR fragment). (B) Levels of RNA encoding Dsc1, Dsc2 and Dsc3 were compared for a further seven matched pairs by quantitative RT–PCR.