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. 2006 Jul 11;95(3):314–321. doi: 10.1038/sj.bjc.6603262

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Copyright © 2006 Cancer Research UK

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Double labelling fluorescent confocal microscopy analysis of coexpression of putative phase-specific markers. (A) There was negligible coexpression of cyclin D1 and cyclin A. (B) In dysplasia there was focal coexpression of cytoplasmic cyclin B1 (green) and cyclin A (red), the latter showing a nuclear and cytoplasmic distribution. An area of maximal coexpression is illustrated here. (C) In SCC, cytoplasmic cyclin B1 (green) was coexpressed by relatively few cyclin A positive cells (red). (D) Rare cells in SCC (arrow) showed nuclear expression of phosphohistone H3 (red) plus cytoplasmic staining for cyclin B1 (green), in keeping with cells at the G2/M transition.