Figure 1.

DNA fragmentation and [³H]thymidine incorporation in response to the treatment of PL, Dox and PL plus Dox. (A) and (B) LNCaP or PrEC cells were cultured in the growth medium in the presence of different concentrations of PL, Dox, or PL plus Dox. After treatment for 48 h, the percentage of cells with fragmented DNA was analysed by a flow cytometer. Error bars represent the s.d. over five independent experiments. (C) Following serum-starvation (0.5% serum) for 48 h, the cells were refed with growth medium containing 10% serum and [³H]thymidine (2 μCi ml⁻¹) in the presence or absence of PL, Dox or both for 24 h. Subsequently, trichloroacetic acid-insoluble radioactivity was determined. Error bars represent the s.d. from five independent experiments.