Table 2. Cell cycle modulation after drug treatments, followed by 24-h culture in drug-free medium. Values (%) are means from three independent experiments and the differences (Δ) are calculated with respect to controls.

Cell lines	Treatment	G1 phase	(ΔG1)	S-phase	Δ(S)	G2/M phase	Δ(G2/M)
	Control	70.50		27.16		2.34
	Gemcitabine (1 h)	49.20	−21.30	46.57	+19.41	4.32	+1.98
T24	Pemetrexed (24 h)	57.05	−13.35	39.28	+12.12	3.67	+1.33
	Gemcitabine-pemetrexed	72.95	+2.45	23.76	−3.4	3.29	+0.95
	Pemetrexed-Gemcitabine	74.49	+3.99	16.80	−10.36	8.71	+6.37
	Control	44.32		39.46		16.22
	Gemcitabine (1 h)	57.94	+13.62	33.46	−6.00	8.60	−7.62
J82	Pemetrexed (24 h)	37.95	−6.37	51.34	+11.88	10.71	−5.51
	Gemcitabine-pemetrexed	28.90	−15.42	61.20	+21.74	9.90	−6.32
	Pemetrexed-Gemcitabine	24.49	−19.83	61.12	+21.66	14.38	−1.84