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. 2006 Aug 15;95(4):548–553. doi: 10.1038/sj.bjc.6603303

Table 2. PTCH PCR primers.

Exon number Primer name Primer sequence (5′ → 3′)
Sequencing primers
 1 PTCH Ex 01F TGG AAG GCG CAG GGT CTG ACT
  PTCH Ex 01 R CGA TCC CAA AGA GTT AGA GGA
 2 PTCH Ex 02F CTG CGG CCC GGC TTT ATG AC
  PTCH Ex 02R GTG TGC GCT GGC GAA TAT CTC TAT C
 3 PTCH Ex 03F ACT GCT CAC ACA TCA GCC AGT CTC AT
  PTCH Ex 03R GCA TTT CCA GGG CAA CTT CAT TTA CTA
 4–5 PTCH Ex 04–05F GCT GGG TCT CTA CTT GGC AAA AGC
  PTCH Ex 04–05R CCC GAC TAT TCA CTC AAA AAA TGC ACA
 6 PTCH Ex 06F ATT TGT TTT GAT GCC AGA GTC CCA GA
  PTCH Ex 06R GGC TAA TGG GAG GTG TAT GGC AAA TC
 7 PTCH Ex 07F AAG ATT TGC CAT ACA CCT CCC ATT AGC
  PTCH Ex 07R AAT TCC CCA CAA GGT GCT TTT TCA A
 8 PTCH Ex 08F GGA AAC ATG TGC TCA CAG AGA AGG AAA
  PTCH Ex 08R TCC CAT CAA GTT CCC AGA ATT GCA
 9 PTCH Ex 09F CCC TGC CCT GGA ATC ACG TAG AAC
  PTCH Ex 09R GAA GCA GGA GCA GTC ATG GAA AAG TAA
 10 PTCH Ex 10F TTT GCC GTT TGC CTA CCT TTG ACT C
  PTCH Ex 10R CGG TGA GAA GGA CAC ACA GCA CAC
 11 PTCH Ex 11F AGG TGC TGG TGG CAG AGT CCT AAC TA
  PTCH Ex 11R GCA GCC AGT GAC ACA TCA TCT GAC AT
 12 PTCH Ex 12F CTG CCA CGT ATC TGC TCA CAC AGT C
  PTCH Ex 12R CAC CCA GTT AAA CAG AGC CTC AAA CAC
 13 PTCH Ex 13F CAC GGT TTC AAA TGC TTC AAG AGG A
  PTCH Ex 13R CAA ACC CCG TTA CCC ACA TTC CTT
 14 PTCH Ex 14F CAG GCG ATG AAC CAG GTG ATG TTA T
  PTCH Ex 14R GAA GCA ATC TGA TGA ACT CCA AAG GTT
 15 PTCH Ex 15F TTG TCC AGG AAG AGT CAG TGG TGC TC
  PTCH Ex 15R GTT GAA GCT GAA CAC GCA AAA GAC C
 16 PTCH Ex 16F CCC TGC CCT GCT CAG TCT CCT C
  PTCH Ex 16R CTG GCA TGA GGT CAC ACA ATT AGC TG
 17 PTCH Ex 17F GCC AGT GAT TGC ATC CTC CGA TAA
  PTCH Ex 17R CCA TTA CAC ATC CTC GTC TCC CAG AG
 18 PTCH Ex 18F CCT CAC AAA GAA TGA CTG CTG GAA GAT
  PTCH Ex 18R CCA GAG GCC CAG ACA TAA ACA AAA CTT
 19 PTCH Ex 19F AAG GTT CCC ACT TGG AGA CAA ACA GAG
  PTCH Ex 19R TGA ATT AGG CAG TAA AGG CAG TGT CCA
 20 PTCH Ex 20F TAC GTC AAC ACC AAA TAT GAC CCA GTG
  PTCH Ex 20R TCT GCC TCA GCC TCC CAA GTA GC
 21 PTCH Ex 21F TGA ATG TGA ACT GCG GTT GGA TAA CA
  PTCH Ex 21R CCA GTA CAC CGA AGA GGA AAA CAG ACA
 22 PTCH Ex 22F CCC CTG AAA AAT ACC GTG CTT TGA G
  PTCH Ex 22R ATC TGC CTG TGT GAT GTG CTG CTC
 23 PTCH Ex 23F GGG TTG ACT GAG TCT TTG GTG AAA CC
  PTCH Ex 23R TTG TCC TCC TCT TTG CCT GGC TCT A
     
Quantitative PCR primer
  PTCHqex1F CCA AAG AGT TAG AGG AGG GAA GAG AAA GT
  PTCHqex1R CTA TCT GCA CCG GCC CAG CTA C
  PTCHqex4F GCT GGG TCT CTA CTT GGC AAA AGC
  PTCHqex4R TTT CCA CTG CCT AAT AAA ATG AAA AGC
  PTCHqex15F AAG AAA ACA AAC AGC TTC CCA AAA TGT
  PTCHqex15R GTT GAA GCT GAA CAC GCA AAA GAC C
  PTCHqex23F TCC AGC CAG CCG TGT CAG AGA
  PTCHqex23R TTC CAC CCA CAA AAG AAA AGC CTG T

NBCCS=nevoid basal cell carcinoma syndrome; PTCH=patched.