Table 2. PTCH PCR primers.
| Exon number | Primer name | Primer sequence (5′ → 3′) |
|---|---|---|
| Sequencing primers | ||
| 1 | PTCH Ex 01F | TGG AAG GCG CAG GGT CTG ACT |
| PTCH Ex 01 R | CGA TCC CAA AGA GTT AGA GGA | |
| 2 | PTCH Ex 02F | CTG CGG CCC GGC TTT ATG AC |
| PTCH Ex 02R | GTG TGC GCT GGC GAA TAT CTC TAT C | |
| 3 | PTCH Ex 03F | ACT GCT CAC ACA TCA GCC AGT CTC AT |
| PTCH Ex 03R | GCA TTT CCA GGG CAA CTT CAT TTA CTA | |
| 4–5 | PTCH Ex 04–05F | GCT GGG TCT CTA CTT GGC AAA AGC |
| PTCH Ex 04–05R | CCC GAC TAT TCA CTC AAA AAA TGC ACA | |
| 6 | PTCH Ex 06F | ATT TGT TTT GAT GCC AGA GTC CCA GA |
| PTCH Ex 06R | GGC TAA TGG GAG GTG TAT GGC AAA TC | |
| 7 | PTCH Ex 07F | AAG ATT TGC CAT ACA CCT CCC ATT AGC |
| PTCH Ex 07R | AAT TCC CCA CAA GGT GCT TTT TCA A | |
| 8 | PTCH Ex 08F | GGA AAC ATG TGC TCA CAG AGA AGG AAA |
| PTCH Ex 08R | TCC CAT CAA GTT CCC AGA ATT GCA | |
| 9 | PTCH Ex 09F | CCC TGC CCT GGA ATC ACG TAG AAC |
| PTCH Ex 09R | GAA GCA GGA GCA GTC ATG GAA AAG TAA | |
| 10 | PTCH Ex 10F | TTT GCC GTT TGC CTA CCT TTG ACT C |
| PTCH Ex 10R | CGG TGA GAA GGA CAC ACA GCA CAC | |
| 11 | PTCH Ex 11F | AGG TGC TGG TGG CAG AGT CCT AAC TA |
| PTCH Ex 11R | GCA GCC AGT GAC ACA TCA TCT GAC AT | |
| 12 | PTCH Ex 12F | CTG CCA CGT ATC TGC TCA CAC AGT C |
| PTCH Ex 12R | CAC CCA GTT AAA CAG AGC CTC AAA CAC | |
| 13 | PTCH Ex 13F | CAC GGT TTC AAA TGC TTC AAG AGG A |
| PTCH Ex 13R | CAA ACC CCG TTA CCC ACA TTC CTT | |
| 14 | PTCH Ex 14F | CAG GCG ATG AAC CAG GTG ATG TTA T |
| PTCH Ex 14R | GAA GCA ATC TGA TGA ACT CCA AAG GTT | |
| 15 | PTCH Ex 15F | TTG TCC AGG AAG AGT CAG TGG TGC TC |
| PTCH Ex 15R | GTT GAA GCT GAA CAC GCA AAA GAC C | |
| 16 | PTCH Ex 16F | CCC TGC CCT GCT CAG TCT CCT C |
| PTCH Ex 16R | CTG GCA TGA GGT CAC ACA ATT AGC TG | |
| 17 | PTCH Ex 17F | GCC AGT GAT TGC ATC CTC CGA TAA |
| PTCH Ex 17R | CCA TTA CAC ATC CTC GTC TCC CAG AG | |
| 18 | PTCH Ex 18F | CCT CAC AAA GAA TGA CTG CTG GAA GAT |
| PTCH Ex 18R | CCA GAG GCC CAG ACA TAA ACA AAA CTT | |
| 19 | PTCH Ex 19F | AAG GTT CCC ACT TGG AGA CAA ACA GAG |
| PTCH Ex 19R | TGA ATT AGG CAG TAA AGG CAG TGT CCA | |
| 20 | PTCH Ex 20F | TAC GTC AAC ACC AAA TAT GAC CCA GTG |
| PTCH Ex 20R | TCT GCC TCA GCC TCC CAA GTA GC | |
| 21 | PTCH Ex 21F | TGA ATG TGA ACT GCG GTT GGA TAA CA |
| PTCH Ex 21R | CCA GTA CAC CGA AGA GGA AAA CAG ACA | |
| 22 | PTCH Ex 22F | CCC CTG AAA AAT ACC GTG CTT TGA G |
| PTCH Ex 22R | ATC TGC CTG TGT GAT GTG CTG CTC | |
| 23 | PTCH Ex 23F | GGG TTG ACT GAG TCT TTG GTG AAA CC |
| PTCH Ex 23R | TTG TCC TCC TCT TTG CCT GGC TCT A | |
| Quantitative PCR primer | ||
| PTCHqex1F | CCA AAG AGT TAG AGG AGG GAA GAG AAA GT | |
| PTCHqex1R | CTA TCT GCA CCG GCC CAG CTA C | |
| PTCHqex4F | GCT GGG TCT CTA CTT GGC AAA AGC | |
| PTCHqex4R | TTT CCA CTG CCT AAT AAA ATG AAA AGC | |
| PTCHqex15F | AAG AAA ACA AAC AGC TTC CCA AAA TGT | |
| PTCHqex15R | GTT GAA GCT GAA CAC GCA AAA GAC C | |
| PTCHqex23F | TCC AGC CAG CCG TGT CAG AGA | |
| PTCHqex23R | TTC CAC CCA CAA AAG AAA AGC CTG T | |
NBCCS=nevoid basal cell carcinoma syndrome; PTCH=patched.