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. 2006 Jul 25;95(4):485–495. doi: 10.1038/sj.bjc.6603273

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Copyright © 2006 Cancer Research UK

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Photodynamic therapy is not enhanced if MTX is administered after ALA and light. Cells were first subjected to ALA-PDT treatment (4 h of ALA 0.3 mM, followed by exposure to graduated light doses) and plated for colony formation assay. Subsequently, one set of plates was exposed to MTX for 72 h, the other to medium only. Colonies were counted at 13 days. (A) Colony formation assay, single experiment. (B) Colony formation assay, pooled experiments. Mean of four experiments±s.e.m.