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. 2006 Aug 1;95(4):496–505. doi: 10.1038/sj.bjc.6603283

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Copyright © 2006 Cancer Research UK

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Immunostaining of senescence effectors in dysplastic naevi. (A) lesions from normal patients. Top and middle, MART1 shows naevus cells throughout basal epidermis in this lesion, yet there is little or no p16, little p53 and no p21 detected in parallel sections. Scale bar, 100 μm. Bottom left, detail of p53 immunostaining in same lesion showing a few positive nuclei (arrowheads). Scale bar, 50 μm. Bottom right, detail of p16 reaction in a different dysplastic naevus. Scale bar, 50 μm. Most naevus cells here are strongly positive for p16, yet the reaction appears generally cytoplasmic (arrowheads: unstained nuclei). Arrow: other parts of this naevus had little or no p16. (B) lesion from a patient lacking active p16 because of two germline mutations. MART1 and p16 immunostaining (top) show similar patterns: nearly all naevus cells were prominently positive for p16. Many naevus cells are also positive for p53 and p21 (middle). Scale bar, 100 μm. Bottom left: p16 reaction is cytoplasmic only (arrowheads: unstained nuclei). Scale bar, 30 μm. Bottom right: p53-positive naevus cells in dermis, in same lesion, surrounded by pigmented macrophages (arrows) and lymphocytes (arrowheads). Scale bar, 50 μm.