Figure 5.

Infection of Raji cured cells by ISVP, or by reovirions in the presence of chymotrypsin. (A) Cytopathic effects. Raji parental or cured cells were exposed to ISVP, reovirus or reovirus in the presence of chymotrypsin (CHT) at an MOI of 20 PFU cell⁻¹, and photomicrographs were taken at 72 h postinfection. Original magnification × 400. (B) Reovirus protein synthesis in infected Raji parental and cured cells. Cells were infected with ISVP, reovirus or reovirus in the presence of CHT, and pulse-labelled with [³⁵S]methionine for 6 h at 18 h postinfection. The cells were then harvested and lysed, and reovirus proteins were immunoprecipitated from an aliquot of the lysate using a rabbit polyclonal anti-reovirus antibody, followed by SDS–PAGE. The three size classes of reovirus proteins (λ, μ and σ) are indicated on the left. (C) Viability of Raji parental and cured cells infected with ISVP, reovirus or reovirus in the presence of CHT. WST-1 assay was carried out on cells from three independent wells at 96 h postinfection. P-values compared with respective uninfected controls are: Left panel, P=0.012 (Raji+Reo), P=0.017 (Raji+ISVP), P=0.61 (Raji cured+Reo) and P=0.026 (Raji cured+ISVP). Right panel, P=0.019 (Raji+Reo), P<0.001 (Raji+Reo+CHT), P=0.98 (Raji cured+Reo) and P=0.0013 (Raji cured+Reo+CHT). ^*Indicates statistical significance.