Figure 6.

Infection of Raji and Raji cured cells in the presence of E64. (A) Reovirus protein synthesis in infected Raji parental and cured cells treated with E64. Cells were infected with ISVP or reovirus in the presence or absence of E64, and pulse-labelled with [³⁵S]methionine for 6 h at 18 h postinfection. The cells were then harvested and lysed, and reovirus proteins were immunoprecipitated from an aliquot of the lysate using a rabbit polyclonal anti-reovirus antibody, followed by SDS–PAGE. The three size classes of reovirus proteins (λ, μ and σ) are indicated on the left. (B) Intratumoral reovirus therapy of lymphoid tumours in SCID mice exposed to E64 injected i.p. SCID mice were subcutaneously implanted with 1 × 10⁷ cells of Raji parental cells. Following palpable tumour establishment, the tumours received (on day 0) either 1 mg per mouse of E64 in PBS alone (n=5) or 1 h before a single intratumoral injection of 1 × 10⁷ PFUs of live reovirus (n=5) and E64 was re-injected subsequently every second day until day 8. E64 treatments were compared to PBS control and reovirus alone group (n=5). Tumour growth was followed for a period of 12 days and measured two-dimensionally with a caliper. ^*Wilcoxon's signed-rank test (P=0.0379) comparing tumour sizes of reovirus+E64-treated group to reovirus-alone group.