Figure 6.

(A) Recognition of recombinant hTERT by GV1001-specific T-cell clone nos. 22 and 30 from patient no 102 and clone 51 from patient no 105. T cells (50 000 well⁻¹) were stimulated with 50 000 irradiated autologous EBV-transformed B cells as APC for 48 h before labelling and harvesting (see below). The APC were pulsed with either GV1001 (10 μM) or recombinant hTERT (3 μM for clones 22 and 30 and 0.5 μM for clone 51). T cells with APC alone served as negative controls. (B) Recognition of ascites cells from patient 105 by T-cell clone nos 35 and 49 derived from the same patient after vaccination. T cells (50 000 well⁻¹) were stimulated for 48 h with 50 000 irradiated, T-cell depleted (anti-CD3-coated Dynabeads) ascites cells or 50 000 irradiated autologous EBV-transformed B cells with or without GV1001 (25 μM) as positive and negative controls. After 48 h of culture, ³H-thymidine was added and the cultures harvested the next day. Results are expressed as mean c.p.m. of triplicate cultures.