Table 2. Proapoptotic effect of NFV on NSCLC cells (TUNEL-positive cells, %).
|
Day 1
|
Day 2
|
|||||
|---|---|---|---|---|---|---|
| NFV( μ M) | — | 20 | 40 | — | 20 | 40 |
| NCI-H460 | 1.0±0.3 | 19.3±4.4* | 24.9±4.4* | 2.4±0.3 | 33.3±2.9* | 50.4±8.6* |
| A549 | 4.6±1.9 | 24.2±5.2* | 36.6±2.1* | 7.4±2.1 | 36.2±4.0* | 59.1±7.2* |
Cells were exposed to NFV (20 or 40 μM). Apoptotic cells were measured by TUNEL assay on day 1 or day 2 of culture. Significance of difference between control cells and cells cultured with NFV was calculated by paired t-test. Results represent the mean±s.d. of two experiments performed in triplicate.
*
P<0.01. NFV=nelfinavir; TUNEL=terminal deoxyribonucleotide transferase-mediated dUTP nick-end labelling.