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. 2005 Dec 13;94(1):101–107. doi: 10.1038/sj.bjc.6602901

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Copyright © 2006 Cancer Research UK

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Angiogenesis was assessed off-line from video recordings taken three and seven days after implantation of alginate coated breast tumour spheroids. The image analysis package Angiosys, UK was used to quantify vessel length, the number of vessels and the number of junctions surrounding spheroids with and without macrophage at days 3 and 7 after implantation. (n=5 mean±s.e.m.). ^* Denotes P<0.01(vessel number, day3), 0.008 (vessel length day 3), 0.025 (number of junctions day 3), 0.021 (vessel length day 7) when compared to no macrophage group using Mann–Whitney U Test.