Table 1. Primer list.

Assay	Orientation	Nucleotide sequence (5′ → 3′)
Rapid amplification of cloned ends
1	Adaptor primer 1, forward	CCATCCTAATACGACTCACTATAGGGC
1	Gene-specific primer 1, reverse	AATTTGTTGGTCTTGTCCAGCTCTGCTTCGTGCG
2	Adaptor primer 2, forward	ACTCACTATAGGGCTCGAGCGGC
2	Gene-specific primer 2, reverse	GCACCGCGGCTTGAGCGTCTCTCGGAAGTGCGGACTATCGAG
Methylation-sensitive PCR
1	Forward	GATTAAGATCGCGGAAGGGTCG
1	Reverse	AACTCTCGACAACCTCGAACGC
2	Forward	CGTTTTCGGGCGGTTTGAGGGTC
2	Reverse	CCTCTCACAACAACCTCGACCG
Real-time PCR
1	GRAF-F	CAGAACATTGTCATTGAGATCCTAATAGA
1	GRAF-R	GGGCATTGGTGAGAGGCATA
1	GRAF probe	AACCACGAAAAGATATTTAACACCGTGCCCGA
1	ABL-F	CAACACTGCTTCTGATGGCAA
1	ABL-R	CGGCCACCGTTGAATGAT
1	ABL probe	CAACACCCTGGCCGAGTTGGTTCAT