Figure 2.

Combined treatment with TRAIL and ATO has synergistic apoptosis-inducing effect. Cells were treated with sublethal dose of TRAIL (Jurkat: 50 ng ml⁻¹, ML-1: 10 ng ml⁻¹, K562: 250 ng ml⁻¹) in the presence or absence of ATO (500 ng ml⁻¹). (A) Apoptotic cell death was measured 12 h post treatment by annexin V staining. One representative dot plot image from three independent experiments is shown. (B) Western blot analysis of pro-caspase-3 and PARP cleavage in Jurkat cells treated with TRAIL and ATO for 10 h. The figure shows one representative picture of three independent experiments. (C) DEVDase activity in TRAIL- and ATO-treated Jurkat cells. DEVDase activity was measured in whole-cell lysates with a kinetic assay. Enzyme activity was expressed in nmole AMC released per minute by 1 mg total cellular protein. The chart represents the result of three independent experiments ±s.d.