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. 2006 Jan 17;94(3):436–445. doi: 10.1038/sj.bjc.6602933

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Copyright © 2006 Cancer Research UK

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Validation by real-time Q-PCR. A 2 μg portion of RNA extracted from primary culture was retro-transcribed and used for real-time Q-PCR using specific primers for RP-1, MSLN, ATPaseβ1, HoxB7, ST-5, ITPR3, TNFR1, KRT7 and BMP-2 in eight malignant samples and nine NOSE (normal ovarian surface epithelia) (A), A2RP, HSU79271, HoxB9 and SmLIM in six LMP (low malignant potential) samples and nine NOSE (B). Each expression level was normalised to that of the control RNA. Relative fold change expression is the ratio of the 61 NOSE gene expression to that of other samples. Owing to the downregulated profile of ST-5 gene expression, PCR was performed using EOC908 as reference. Green colour represents expression ratio lower than 1, black represents expression ratio equal to 1 and red represents expression ratio higher than 1.