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. 2008 May 16;4(5):e1000066. doi: 10.1371/journal.ppat.1000066

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Benoit et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A and B, Mo (A) and MDM (B) were incubated with AL and NL for 30 min. Mo and MDM were fixed, gold-coated and analyzed by SEM or labeled with BODIPY phallacidin and observed in fluorescence microscopy. Representative micrographs are shown. C and D, the percentage of Mo (C) and MDM (D) that exhibited membrane ruffles or spikes was determined by fluorescence microscopy. E and F, Fluorescent AL and NL were incubated with Mo and MDM (ratio of 5∶1) for different times. Mo and MDM were labeled with BODIPY phallacidin, and the binding of AL and NL was quantified by fluorescence microscopy. The results are expressed as the percentage of Mo (E) and MDM (F) that have bound at least one AL or NL. More than 100 cells were examined by experimental condition. The results represent the mean±SEM of 5 experiments.