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. 2008 May 16;4(5):e1000066. doi: 10.1371/journal.ppat.1000066

Figure 4. Effect of AL binding on the maturation of C. burnetii-phagosomes.

Figure 4

MDM (A), AL-MDM (B) or NL-MDM (C) were stimulated with C. burnetii for 4 h, washed and then cultured for 24 h. Cells were labeled with anti-C. burnetii (Alexa 546), anti-Lamp-1 (Alexa 488) and anti-cathepsin D (Alexa 647) Abs and analyzed under a confocal microscope. A–C, Representative micrographs are shown with expanded images (white rectangle). D, Percentage of C. burnetii phagosomes that colocalized with Lamp-1 and cathepsin D. The results represent the mean±SEM of 4 independent experiments. * p<0.01.