Table 2.
Changes in nuclear methylation between the vegetative and generative pollen cells in developing tobacco
| Immature pollen
|
Mature pollen
|
||||||
|---|---|---|---|---|---|---|---|
| Pollen | Ratio of vegetative to generative nuclei
|
Pollen | Ratio of vegetative to generative nuclei
|
||||
| m5C F | Histone F | m5C/histone | m5C F | Histone F | m5C/histone | ||
| A | 1.50 | 1.26 | 1.19 | K | 5.48 | 0.92 | 5.96 |
| B | 1.42 | 1.52 | 0.93 | L | 5.54 | 1.06 | 5.23 |
| C | 1.32 | 1.36 | 0.97 | M | 5.19 | 0.97 | 5.35 |
| D | 1.27 | 1.26 | 1.01 | N | 5.32 | 1.10 | 4.84 |
| E | 1.26 | 1.28 | 0.98 | O | 4.79 | 0.93 | 5.15 |
| F | 1.36 | 1.51 | 0.90 | P | 5.42 | 1.09 | 4.97 |
| G | 1.25 | 1.22 | 1.02 | Q | 6.05 | 1.37 | 4.42 |
| H | 1.28 | 1.44 | 0.89 | R | 5.47 | 0.93 | 5.88 |
| I | 1.23 | 1.18 | 1.04 | S | 6.49 | 1.44 | 4.51 |
| J | 1.14 | 1.25 | 0.91 | T | 5.57 | 1.04 | 5.36 |
| Mean | 0.98 | 5.17 | |||||
| SD | 0.09 | 0.51 | |||||
“Immature pollen” shows the corrected relative fluorescence values (F) for the generative and vegetative nuclei in postmitotic pollen. “Mature pollen” shows the relative fluorescence values for the generative and vegetative nuclei in mature pollen immediately prior to another dehiscence.