Table 3. Primers and parameters for PCR-single-strand confirmation polymorphism (SSCP) analysis.

Gene	Forward (5′–3′)	Reverse (3′–5′)	Product size (bp)	Number of cycle	Annealing condition
CKMT1 (15q15)
Exon1	CCCTGTTCCGGATCTTATCT	CCGGATTCTCACTTCTACCC	292	35	64°C, 10 s
Exon2	CCCTCCATGGTTACTGGGTA	TGGATCATCAGGGAGACTCTG	264	35	64°C, 10 s
Exon3	TTGAAGTCTGGGGAGGGATT	ACACAGGCAATGCAAATCAG	196	35	64°C, 10 s
Exon4	CAATGCATGCAGGAAGAATG	CTGCTGTTCAGCCTCTGTCA	332	35	64°C, 10 s
Exon5	CCAGATGAGACATGGGCTCT	TCAAGAGGCAAGGCAAGAAT	254	35	64°C, 10 s
Exon6	ATTCTTGCCTTGCCTCTTGA	ATGTCCTGGGTTGGTTGGTA	267	35	64°C, 10 s
Exon7	TAAGGGAGGGTCCAGGGTTA	CTTTCCAAAGGGTCCACTCC	283	35	64°C, 10 s
Exon8	CAGGTTCAGGGCTCTTTCAG	CTTGCCTGCTCATTTCCAAT	344	35	64°C, 10 s
Exon9	ATTGGAAATGAGCAGGCAAG	AGACAGCTGCAGCATAGCAA	343	35	64°C, 10 s