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. 2006 Feb 21;94(5):724–730. doi: 10.1038/sj.bjc.6602990

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Copyright © 2006 Cancer Research UK

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RT–PCR analysis of ADAM-12 and ADAMTS-1 in human tumour samples and normal lung tissues (n=39) and in lung cancer cell lines. (A) mRNA transcripts of total ADAM-12. The expression of ADAM-12 mRNA is significantly higher in tumours (T) than in their control tissues (C). (B) Expression of ADAMTS-1 mRNA. ADAMTS-1 expression is lower in tumour tissues when compared to the corresponding control tissue. L: 200 bp molecular weight (Smart Ladder, Eurogentec, Seraing, Belgium). (C–D) ADAM-12 (left panel) and ADAMTS-1 (right panel) mRNA expression in human lung cancer cell lines. The bottom line represents the 28S RNA. (E–F) Results of quantitative real-time PCR for ADAM-12 and ADAMTS-1, expressed as arbitrary units (AU) normalised to the 18S rRNA (described in the Materials and Methods section).