. 2006 Feb 21;94(5):661–671. doi: 10.1038/sj.bjc.6602996

Table 1. Cell lines used in this study.

Cell line^(source)	Tissue	Tumour type	Genotype confirmation	CDH1 promoter methylation	Protein expression	CDH1 mutation⁽⁹⁾	QPCRa	Flow index MESF
BT474⁽¹⁾	Breast	IDC	+	−	+		192.7
BT483⁽¹⁾	Breast	IDC	NIA	−			226.6
BT549⁽¹⁾	Breast	PIDC	NIA	±	−		3.2
CAMA1⁽¹⁾	Breast	C	+⁽⁷⁾	−	+	+	23.9	17
Du4475⁽¹⁾	Breast	IDC	+	−	+	−	24.5
HBL100⁽¹⁾	Breast	Normal⁽⁸⁾	NIA⁽⁸⁾	+	−		0.0
Hs578T⁽¹⁾	Breast	CS	+	±	−	−	0.0	0
MCF10A⁽¹⁾	Breast	Normal		±			1.0
MCF10F⁽¹⁾	Breast	Normal	+	±	−		0.3
MCF12A⁽¹⁾	Breast	Normal	+	±			10.7
MCF7⁽¹⁾	Breast	IDC	+	−	+	−	201.8	47
MDA-MB-134 VI⁽¹⁾	Breast	IDC	+	−	−	+	3.8
MDA-MB-175 VII⁽¹⁾	Breast	IDC	+	−	+	−	281.1
MDA-MB-231⁽¹⁾	Breast	IDC	+	±	±	−	30.3	18
MDA-MB-231^*(6)	Breast	IDC	⁽⁶⁾	±	−		0.3
MDA-MB-330⁽¹⁾	Breast	IDC	+	−	+	−	99.1
MDA-MB-361⁽¹⁾	Breast	AC	+	−	+	−	243.6
MDA-MB-435s⁽¹⁾	Breast	IDC	+	+	−		0.0	0
MDA-MB-453⁽¹⁾	Breast	AC	+	−		−	6.8
MPE600⁽²⁾	Breast	C	NIA	−	+	+	96.2	108
OCUB-F⁽³⁾	Breast	C	NIA	−	−	+	75.7
SK-BR-3⁽¹⁾	Breast	IDC	+	Del	−	+	0.1	0
SK-BR-5⁽⁴⁾	Breast	C	NIA	−	−	+	20.1	0
SUM185PE⁽⁵⁾	Breast		NIA	−	+		131.2
SUM44PE⁽⁵⁾	Breast	ILC	NIA	−	−	+	5.4
T47D⁽¹⁾	Breast	IDC	+	−	+	−	97.2	121
ZR75-1⁽¹⁾	Breast	IDC	+	−	+	−	284.8	103
LS180⁽¹⁾	Colorectal	AC		−
LS411N⁽¹⁾	Colorectal	C		−
SW480⁽¹⁾	Colorectal	AC		−
SW837⁽¹⁾	Rectal	AC		−

⁽¹⁾American Tissue Type Collection (ATCC); ⁽²⁾Dr F Waldman; ⁽³⁾Riken Gene Bank; ⁽⁴⁾Dr E Stockert; ⁽⁵⁾Dr S Ethier; ⁽⁶⁾a clone spontaneously derived from MDA-MD-231 lacking allele 8 of TPOX; ⁽⁷⁾lost the 9.3 allele of THO1; ⁽⁸⁾HBL100 was originally isolated from a healthy female; however, it turned out to have a Y chromosome; ⁽⁹⁾after van de Wetering et al. (2001).

Normalised real-time PCR SQ values for CDH1 expression; IDC=invasive ductal carcinoma; PIDC=papillary invasive ductal carcinoma; C=carcinoma; CS=carcinosarcoma; ILC=invasive lobular carcinoma; AC=adenocarcinoma; NIA=no information available; (blank)=not determined; Del=promoter deletion.