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. 2006 Apr 25;94(10):1496–1503. doi: 10.1038/sj.bjc.6603103

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Copyright © 2006 Cancer Research UK

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(A) The effect of OPG and neutralising antibodies to osteoclastogenic humoral factors on resorption in PBMC cultures incubated with M-CSF and 10% SK-Mel-29 conditioned medium (CM). The data represent the mean % surface area (SA) lacunar resorption relative to the positive control (PBMC cultures with M-CSF and RANKL). Error bars denote s.e.m. (n=6). (B) The effect of the low and high molecular weight components within SK-Mel-29 CM on human monocyte–osteoclast differentiation. The data represent the mean % surface area (SA) lacunar resorption relative to the positive control (PBMC cultures with M-CSF and RANKL). Error bars denote s.e.m. (n=6). In addition, the mean number of TRAP+ multinucleated cells (MNC) is also demonstrated (n=2).