Table 4. Correlation between gene copy by qPCR and by FISH.
| Gene copy changes |
FISH
|
||
|---|---|---|---|
| qPCR | Nonamplified | Amplifieda | P-valueb |
| All | 22 | 37 | |
| Nonamplified | 13 | 9c | 0.008 |
| Amplified | 9 | 28 | |
| Adenocarcinoma | |||
| Nonamplified | 6 | 1 | 0.004 |
| Amplified | 5 | 18 | |
| Squamous cell carcinoma | |||
| Nonamplified | 7 | 7 | 0.5 |
| Amplified | 4 | 8 | |
a
Amplified tumours were those showing presence of tight hTERT gene clusters or hTERT to chromosome ratio of ⩾2, or ⩾15 copies per cell in ⩾10% of tumour cells, as defined by Cappuzzo et al (2005).
b
P-values calculated using the two-sided Fisher's exact test.
c
One case was a large-cell carcinoma.
qPCR=quantitative polymerase chain reaction; FISH=fluorescence in situ hybridisation.