Table 3.
Steady-state kinetic parameters for the peroxidase activity of wild-type and variant forms of horse heart myoglobin [sodium phosphate buffer (0.1 M), pH 6, 25°C]
| Protein |
k1
|
k3
|
||
|---|---|---|---|---|
| (M−1 s−1) | k1(variant)/k1(wild-type) | (M−1 s−1) | k3(variant)/k3(wild-type) | |
| Wild-type Mb | 5.4(2) × 102 | 1 | 8.8(1) × 103 | 1 |
| F46L | 1.32(3) × 103 | 2.4 | 4.0(4) × 103 | 0.5× |
| F46L/I107F | 2.67(2) × 103 | 4.9 | 4.1(3) × 103 | 0.5× |
| T39I/F46L/I107F | 1.02(2) × 104 | 18.9 | 1.9(9) × 104 | 2.2× |
| T39I/K45D/F46L/I107F | 1.34(5) × 104 | 24.7 | 1.4(3) × 104 | 1.6× |