Table 5.
Parameters for O2 and CO binding to wild-type and variant forms of horse heart myoglobin
| Protein | O2
|
CO
|
||||
|---|---|---|---|---|---|---|
| kon × 10−6 (M−1 s−1) | koff (s−1) | KO2 × 10−6 (M−1) | kon × 10−6 (M−1 s−1) | koff × 102 (s−1) | KCO × 10−7 (M−1) | |
| Wild-type Mb | 22.3 | 24.2 | 0.92 | 0.51 | 3.51 | 1.45 |
| F46L | 17.1 | 44.3 | 0.39 | 0.62 | 5.98 | 1.04 |
| F46L/I107F | 17.1 | 25.2 | 0.68 | 0.55 | 3.31 | 1.66 |
| T39I/F46L/I107F | 19.6 | 23.5 | 0.83 | 0.76 | 4.8 | 1.58 |
| T39I/K45D/F46L/I107F | 22.2 | 24.3 | 0.91 | 0.9 | 5.08 | 1.77 |