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. 2006 May 9;94(11):1663–1671. doi: 10.1038/sj.bjc.6603148

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Copyright © 2006 Cancer Research UK

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In vitro translation of pcDNA3.1+PIF. (A) Gel of ³⁵S-methionine labelled in vitro translation reaction using the coupled T7 RNA poymerase and rabbit reticulocyte lysate system. DNA templates used were from left to right, pcDNA3.1+ (empty vector), pcDNA3.1+PIF and luciferase. Translation of PIF from the pcDNA3.1+PIF plasmid resulted in an 11 kDa product (middle lane). The 61 kDa product of the luciferase control gene can be seen in the right-hand lane. (B) Addition of CPMM resulted in the dose-dependent production of a 2.5 kDa band in translation reactions in which pcDNA3.1+PIF was used as a template. (C) Translation of the N32Q, N44Q and N32Q N44Q mutants in the presence of 1.2 μl per reaction of CPMM resulted in products of molecular weight identical to the translation product of wild-type PIF/dermcidin.