Table 2. Characteristics of primary OS cells.
| Code | Age | M/F | Diagnose | p53 statusa (PG13-Luc/MG15-luc) | Population doubling time (days) |
|---|---|---|---|---|---|
| OS-5A | 7 | F | High grade OS | 3.1 | >7 |
| OS-6 | 25 | M | High grade OS | 1.2 | >7 |
| OS-8 | 6 | M | High grade OS | 10.5 | 3.4±0.7 |
| OS-11 | 16 | F | High grade OS | 4.8 | 3.6±1.0 |
| OS-11A | 1.5 | 4.9±1.2 | |||
| OS-12 | 13 | F | High grade OS | 9.8 | 4.4±0.7 |
| OS-12A | 7.6 | 4.2±0.8 | |||
| OS-16 | 15 | F | High grade OS | 5.3 | 1.4±0.5 |
a
Functional p53 status was determined by measuring relative luciferase expression after PG13-Luc transfection compared to MG15-Luc transfection. Scores: ratio 0.5–2.0, deficient; ratio 2–10, heterogeneous; and ratio>10, functional.
OS=osteosarcoma.